Oncology FISH Analysis - MLL: 11q23

Clinical Use

Use

Oncology FISH Analysis – MLL:11q23 is a Fluorescence In Situ Hybridization (FISH) test designed to detect a common chromosomal rearrangement associated with acute myeloid leukemia (AML). AML is a type of leukemia affecting mostly adults that commonly develops from undifferentiated white blood cells, originating from bone marrow and traveling into the blood stream. It may spread to other parts of the body affecting the central nervous system, liver, lymph nodes, spleen, and testicles. This rearrangement, known as the KMT2A rearrangement, is associated with a poor prognosis, found in 10% of all AML cases and 50% of infants with AML. Results must be interpreted in the context of relevant clinical and pathological data.

Special Instructions

This test is indicated for patients with clinical suspicion or confirmed diagnosis of acute myeloid leukemia, prognostic evaluation of anomalies in diagnosed individuals, cases where prior cytogenetic testing was inconclusive, and monitoring therapy response. Specimens should be shipped in an insulated container and must arrive at the laboratory within 48 hours post-collection for optimal analysis.

Limitations

This panel only tests for rearrangements involving the 11q23/KMT2A loci. Rearrangements with other partner chromosomes cannot be determined from this assay.

Test Details

Methodology

Chromosomal / Cytogenetics (FISH)

Biomarkers

2 components

Primary Component • FISHFISH Analysis • FISH

Result Turnaround Time

3-5 days

Specimen

Bone Marrow (Fresh)

Volume

3-5 cc

Minimum Volume

3-5 cc

Container

Sodium Heparin (green-top) tube

Collection Instructions

Draw bone marrow in a Sodium Heparin (green-top) tube(s) and send 3-5 cc (Adults/Children) or 2 cc (Infant< 2 years)

Storage Instructions

Ship at ambient temperature (18-25°C/64-77°F) in an insulated container by overnight courier. Specimen should arrive in the laboratory within 48 hrs of collection. Do not heat or freeze.