17-OH Progesterone, LC/MS, ACTH Stimulation
Also known as: 17-OHP ACTH Stimulation
Use
This test may involve significant risk and should be performed only by qualified personnel who are familiar with the test and who have taken adequate precautions to protect the safety of the patient. Every effort has been made to ensure accuracy in these recommendations, but clinicians must use their judgment and refer to specific pharmaceutical resources to determine appropriate drug dosages for their patients.
Special Instructions
Draw blood for baseline 17-OH progesterone. Inject cosyntropin 250 μg IM or IV (if IV, dilute cosyntropin in 2 to 5 mL of sterile saline and inject over two minutes). Draw blood for stimulated 17-OH progesterone 60 minutes after. See the online Endocrine Appendix: ACTH Stimulation for expected values for various steroids at baseline and after ACTH stimulation.
Limitations
The test may involve additional confirmatory or reflex tests which could extend the turnaround time. The test's interpretative values might vary depending on the methodology and patient population.
Methodology
Mass Spectrometry
Biomarkers
LOINC Codes
- 27996-8
- 14114-3
Result Turnaround Time
3-7 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL (each specimen)
Minimum Volume
0.5 mL
Container
Red-top tube, gel-barrier tube or lavender-top (EDTA) tube
Collection Instructions
If tube other than gel-barrier tube is used, transfer separated serum or plasma to a plastic transport tube. Note: Label each tube with patient's name, and date and time of collection. Submit specimens simultaneously on the same test request form.
Storage Instructions
Refrigerate.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 7 days |
| Frozen | 14 days |