Immune Complexes, C3d Circulating

Also known as: C3d, Complement c3d, Complement c3dg, Complement ic3b, Raji cell, Quantitative

Clinical Use

Order Test

Use

For the measurement of C3d-bound circulating immune complexes (CIC) present in human plasma or serum.

Special Instructions

Not provided.

Limitations

False-positives may occur in the presence of antilymphocyte antibodies. This assay does not detect noncomplement fixing CIC, and occasionally, positive specimens may not confirm due to contamination, heat inactivation, or presence of human IgG antibodies binding to mouse IgG. The assay's design means specimens demonstrating false positives may still have concomitant CIC that would confirm positive under other conditions.

Test Details

Methodology

Immunoassay (ELISA)

Biomarkers

C3d-bound immune complexes

Analyte

C3d Immune Complexes

Concentration / Level • Quantitative (Continuous) (e.g., copies/mL, % expression)

LOINC Codes

Result Codes

10864-7 - IC SerPl Raji Cell-aCnc

Result Turnaround Time

4-8 days

Specimen

Plasma

Volume

1 mL

Minimum Volume

0.1 mL

Container

Red-top tube or gel-barrier tube; lavender-top (EDTA) tube is also acceptable.

Collection Instructions

Transfer specimen to a plastic transport tube before freezing. To avoid delays, submit separate frozen specimens for each test requested.

Storage Instructions

Freeze at -70°C or store on dry ice within six hours of collection. Specimen is stable for 14 days if stored at -70°C. Storage at -20°C will yield erroneous results.