Immunofixation (IFE) and Protein Electrophoresis, 24-Hour Urine
Also known as: Bence Jones Protein Identification, 24-Hour Urine, Globulins, 24-Hour Urine, IFE/Protein Electrophoresis, 24-Hour Urine, Myeloma Proteins, 24-Hour Urine
Use
Useful in characterizing the species of protein or proteins in cases of proteinuria. Techniques can identify albumin, γ globulin, γ globulin fragments (light chains, Bence Jones proteins). Of value in the study of monoclonal gammopathies, lymphoproliferative diseases, including lymphoma; special application to multiple myeloma, macroglobulinemia of Waldenström and amyloidosis. Monoclonal gammopathy (M spike) work-up.
Special Instructions
The request form must include the patient's age and clinical diagnosis. When submitting a specimen for this test, be sure to label it appropriately and complete all necessary documentation for accurate processing.
Limitations
While the test is proficient at identifying and characterizing proteins in urine, limitations may arise from improper sample collection or storage, potentially affecting the accuracy of results. False negatives could occur in cases where protein concentration is below the test's sensitivity threshold. Additionally, external factors such as specimen handling or exposure to preservatives may lead to test rejection.
Methodology
Other
Biomarkers
LOINC Codes
- 95801-7
- 2888-6
- 2889-4
- 13992-3
- 13990-7
- 13993-1
- 13994-9
- 13989-9
- 42483-8
- 42482-0
- 13440-3
- 8251-1
- 11502-2
Result Turnaround Time
3-5 days
Related Documents
For more information, please review the documents below
Specimen
Urine
Volume
30-mL aliquot
Minimum Volume
11-mL aliquot
Container
12-mL plastic transport tube (Labcorp No. 23597) without preservative
Collection Instructions
Collect 24-hour urine; mix well.
Storage Instructions
Refrigerated (preferred) or room temperature
Causes for Rejection
Preservative added
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |