Legionella pneumophila, DFA
Also known as: Legionella Antigen, FA Smear for Legionella pneumophila
Use
Determine the presence of Legionella pneumophila organisms in direct FA smear of specimen, providing rapid diagnosis
Special Instructions
Avoid contamination with normal flora from skin or other body surfaces. Store and transport the specimen at 2°C to 8°C within 24 hours of collection to ensure accuracy of test results.
Limitations
This DFA test only detects Legionella pneumophila and does not include other Legionella species. False-positive reactions have been observed in cases of pleuropulmonary tularemia and Campylobacter infection. Specimens received in conditions such as swabbing, inappropriate transport devices, or prolonged delays might be rejected.
Methodology
Immunoassay (Direct fluorescent antibody (DFA))
Biomarkers
LOINC Codes
- 89594-6
- 89594-6
Result Turnaround Time
2-5 days
Related Documents
For more information, please review the documents below
Specimen
Other
Volume
1 mL lung exudate or 1 cc lung biopsy
Minimum Volume
Not provided
Container
Sterile leakproof container or slides
Collection Instructions
Contamination with normal flora from skin or other body surfaces should be avoided.
Storage Instructions
Refrigerate. Maintain specimen at 2°C to 8°C, and transport to the laboratory within 24 hours of collection.
Causes for Rejection
Swab specimen; inappropriate specimen transport device; improper labeling; specimen received after prolonged delay (usually more than 72 hours); environmental water samples such as air conditioning/cooling towers