T-Lymphocyte Helper/Suppressor Profile
Also known as: Helper:Suppressor Ratio, T4:T8 Analysis
Use
This test enumerates CD4 and CD8 T-cells which are crucial for monitoring immune function in HIV-1 seropositive patients. The progression of HIV-1 infection typically results in a decrease in CD4 T cells, an increase in CD8 T cells, and a decrease in the CD4:CD8 ratio, indicative of progressive destruction of immune function. These measurements are used for prognostic purposes, monitoring disease progression, and evaluating the effectiveness of retroviral therapy.
Special Instructions
Not provided.
Limitations
The accurate enumeration of CD4+ and CD8+ T-cells depends on using appropriate controls and calibration in flow cytometry. Specimens must be processed within the specified time frame (48 hours) to ensure accurate results. Hemolysis, clotted or contaminated specimens, and specimens that are refrigerated or frozen are causes for rejection. Measurement variability may be introduced if processing steps are not adhered to stringently.
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
LOINC Codes
- 65759-3 - T-cell subsets CD4+CD8 pnl Bld
- 8122-4 - CD3 Cells # Bld
- 24467-3 - CD3+CD4+ Cells # Bld
- 14135-8 - CD3+CD8+ Cells # Bld
- 8124-0 - CD3 Cells NFr Bld
- 8123-2 - CD3+CD4+ Cells NFr Bld
- 8101-8 - CD3+CD8+ Cells NFr Bld
- 54218-3 - CD3+CD4+ Cells/CD3+CD8+ Cll Bld
- 6690-2 - WBC # Bld Auto
- 789-8 - RBC # Bld Auto
- 718-7 - Hgb Bld-mCnc
- 4544-3 - Hct VFr Bld Auto
- 787-2 - MCV RBC Auto
- 785-6 - MCH RBC Qn Auto
- 786-4 - MCHC RBC Auto-EntMCnc
- 788-0 - Erythrocyte DistWidth Bld Auto
- 777-3 - Platelet # Bld Auto
- 770-8 - Neutrophils NFr Bld Auto
- 736-9 - Lymphocytes NFr Bld Auto
- 5905-5 - Monocytes NFr Bld Auto
- 713-8 - Eosinophil NFr Bld Auto
- 706-2 - Basophils NFr Bld Auto
- 75652-8 - Imm Lymphs Bld Ql
- 751-8 - Neutrophils # Bld Auto
- 731-0 - Lymphocytes # Bld Auto
- 742-7 - Monocytes # Bld Auto
- 711-2 - Eosinophil # Bld Auto
- 704-7 - Basophils # Bld Auto
- 71695-1 - Imm Granulocytes NFr Bld Auto
- 53115-2 - Imm Granulocytes # Bld Auto
- 58413-6 - nRBC Bld Auto-Rto
- 18314-5 - Morphology Bld-Imp
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
Fill tube(s) to capacity.
Minimum Volume
Not provided
Container
Lavender-top (EDTA) tube and yellow-top (ACD-A or ACD-B) tube
Collection Instructions
Invert tube 8 to 10 times immediately after collection.
Storage Instructions
Maintain specimen at room temperature. Do not freeze or refrigerate.
Causes for Rejection
Hemolysis; specimen refrigerated or frozen; clotted specimen; contaminated specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Yellow-top (ACD-A) or (ACD-B) tube: 2 days; Lavender-top (EDTA) tube: 1 day |
| Refrigerated | Unstable |
| Frozen | Unstable |
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