Prothrombin Antibody (IgG)
Use
This test is intended for the detection and semi‑quantitation of IgG anti‑prothrombin (aPT) antibodies. Antiphospholipid (aPL) antibodies are a heterogeneous group of antibodies that bind to protein‑phospholipid complexes; aPT antibodies may be detected in patients with autoimmune diseases (e.g., systemic lupus erythematosus) and/or antiphospholipid syndrome (APS). The aPT antibody concentration values obtained from this assay serve as an aid to diagnosis only. If results are negative but clinical history suggests APS, further testing for APS criteria antibodies (IgG and IgM beta‑2‑glycoprotein I, cardiolipin antibodies, lupus anticoagulant) is recommended, with repeat testing in at least 12 weeks to demonstrate persistence of antibodies.
Special Instructions
Not provided.
Limitations
Not provided.
Methodology
Immunoassay
Biomarkers
IgG anti‑prothrombin antibody
Other
Result Turnaround Time
1-8 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
1 mL (preferred)
Minimum Volume
0.3 mL
Container
3.2% sodium citrate (light blue‑top) tube (preferred); serum acceptable
Collection Instructions
Collect plasma via venipuncture, centrifuge immediately at 1500 × g for 10 minutes; separate supernatant carefully to avoid platelet contamination; repeating centrifugation may be advisable
Storage Instructions
Transport frozen on dry ice
Causes for Rejection
Gross hemolysis; specimen received at room temperature
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 14 days |
| Frozen | 21 days |
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