Smooth Muscle Actin (SMA) by Immunohistochemistry
Also known as: SMA IHC
Use
This immunohistochemistry stain (Smooth Muscle Actin, SMA) is used as a technical 'stain and return' diagnostic service to detect smooth muscle actin expression in tissue or cells, aiding in histopathologic evaluation. It is performed in a clinical laboratory setting as a Laboratory Developed Test (LDT).
Special Instructions
Ordering requires submission by electronic request or use of ARUP Immunohistochemistry Stain Form (#32978) with ARUP client number. A tissue transport kit (ARUP supply #47808) is required for transport of tissue blocks or slides. Precise collection, slide preparation (min 2 slides, 3–5 μm thick, positively charged), and transport instructions are specified; avoid oven baking precut slides.
Limitations
Specimens unacceptable if non-representative tissue type or depleted. Slides must not be oven baked. Frozen specimens are unacceptable. Specimens must be formalin-fixed (10% neutral buffered) and paraffin-embedded. Frozen specimens cannot be used.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 94736-6
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Tissue block or slides in tissue transport kit (ARUP supply #47808)
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen (cells into cellblock). For slides: 5 unstained (3‑ to 5‑micron thick), positively charged slides (min: 2). Do not oven bake precut slides.
Storage Instructions
Room temperature or refrigerated; ship in cooled container during summer months.
Causes for Rejection
Non‑representative tissue type; depleted specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |