Thyroid Transcription Factor (TTF-1) by Immunohistochemistry
Also known as: TTF1 IHC
Use
Thyroid Transcription Factor 1 (TTF-1) by Immunohistochemistry is a technical service offered for the detection of TTF-1 expression in tissue samples. This test is valuable in differentiating primary lung adenocarcinomas from metastatic lung tumors, as TTF-1 is typically expressed in primary lung and thyroid neoplasms. It aids in the histological diagnosis and subclassification of lung and thyroid cancer cases. The results, derived from stained tissue sections, help pathologists confirm or rule out certain diagnoses based on TTF-1 expression patterns.
Special Instructions
Submit an electronic request for this test. If you do not have electronic ordering capability, use an ARUP Immunohistochemistry Stain Form (form #32978) with an ARUP client number. The test is performed as a stain and return service, meaning only the staining process is conducted and the results are returned to the requesting clinician for interpretation.
Limitations
This immunohistochemical test detects the presence and pattern of TTF-1 expression in tissue sections. However, the interpretation of TTF-1 staining must be done within the clinical context by a qualified pathologist, as staining intensity and pattern may vary. The test does not provide quantitative results or direct information on gene mutations or expression levels beyond the presence of the protein. Furthermore, false positives/negatives may occur due to technical errors or non-representative tissue samples.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 94736-6
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Collect tissue or cells. Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen. If cells, prepare into a cellblock. Transport tissue block or 5 unstained (3- to 5-micron thick sections), positively charged slides in a tissue transport kit.
Storage Instructions
Protect paraffin block and/or slides from excessive heat. Room temperature or refrigerated.
Causes for Rejection
Specimens submitted with non-representative tissue type. Depleted specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |