Thyroid Transcription Factor (TTF-1) by Immunohistochemistry

Also known as: TTF1 IHC

Clinical Use

Use

Thyroid Transcription Factor 1 (TTF-1) by Immunohistochemistry is a technical service offered for the detection of TTF-1 expression in tissue samples. This test is valuable in differentiating primary lung adenocarcinomas from metastatic lung tumors, as TTF-1 is typically expressed in primary lung and thyroid neoplasms. It aids in the histological diagnosis and subclassification of lung and thyroid cancer cases. The results, derived from stained tissue sections, help pathologists confirm or rule out certain diagnoses based on TTF-1 expression patterns.

Special Instructions

Not provided.

Limitations

This immunohistochemical test detects the presence and pattern of TTF-1 expression in tissue sections. However, the interpretation of TTF-1 staining must be done within the clinical context by a qualified pathologist, as staining intensity and pattern may vary. The test does not provide quantitative results or direct information on gene mutations or expression levels beyond the presence of the protein. Furthermore, false positives/negatives may occur due to technical errors or non-representative tissue samples.

Test Details

New York Approved

Methodology

Immunoassay (IHC)

Biomarkers

TTF-1

Protein

IHC

Expression • Categorical (e.g., Positive / Negative / Indeterminate)

LOINC Codes

Result Codes

94736-6 - Tissue block ID Spec

Result Turnaround Time

1-3 days

Specimen

Tissue (FFPE)

Volume

Not provided

Minimum Volume

Not provided

Collection Instructions

Collect tissue or cells. Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen. If cells, prepare into a cellblock. Transport tissue block or 5 unstained (3- to 5-micron thick sections), positively charged slides in a tissue transport kit.