Oncology FISH Analysis - ROS1 Rearrangement

Clinical Use

Use

Oncology FISH Analysis – ROS1 Rearrangement is a Fluorescence In Situ Hybridization (FISH) test designed to detect a common chromosomal rearrangement associated with non-small cell lung cancer (NSCLC). NSCLC is the most common type of lung cancer and can often be confused with common illnesses due to symptoms such as coughing and fatigue. The presence of ROS1-associated rearrangement is significant as it may indicate a better response to ALK-targeted therapy. It is crucial that the results of this test are interpreted alongside relevant clinical and pathological data; this test should not be utilized in isolation for a diagnosis of non-small cell lung cancer.

Special Instructions

For FFPE specimens undergoing this FISH test, the tissues should be fixed in 10% neutral buffered formalin for a span of 6 to 48 hours. The amount of formalin must at least be tenfold the specimen volume. Avoid using decalcification solutions with strong acids. Fixation for less than 6 hours or beyond 48 hours may lead to false negative results.

Limitations

The FISH assay focuses on the rearrangement of the ROS1 loci specifically. It does not identify abnormalities involving other loci, which can limit the breadth of analysis for comprehensive genetic profiling.

Test Details

New York Approved