α2-Macroglobulin, Quantitative
Also known as: Alpha-2 Macroglobulin, AMG
Use
α2-macroglobulin assay is of major significance in the differential diagnosis of nephrotic syndrome, where an elevated α2-macroglobulin:albumin ratio is indicative of postrenal hematuria. In patients with liver cirrhosis and diabetes, the level of α2-macroglobulin is found to be elevated.
Special Instructions
It is important to separate serum from cells within 30 to 60 minutes after collection and transfer the specimen to a plastic transport tube the serum is separated. Use either a red-top tube or gel-barrier tube for collection.
Limitations
Measured levels of α2-macroglobulin can be low in hyperfibrinolytic states, major surgeries, septicemia, and severe hepatic insufficiency, which may affect test results. Patients with acute pancreatitis may also exhibit low serum concentrations correlating with disease severity. Additionally, gross hemolysis and unresolvable gross lipemia can lead to sample rejection.
Methodology
Immunoassay
Biomarkers
LOINC Codes
- 1835-8
- 1835-8
Result Turnaround Time
2-3 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.2 mL (adult), 0.1 mL (pediatric)
Container
Red-top tube or gel-barrier tube
Collection Instructions
Separate serum from cells within 30 to 60 minutes after collection. Transfer specimen to a plastic transport tube.
Storage Instructions
Maintain specimen at room temperature.
Causes for Rejection
Gross hemolysis (>1000 mg/dL); gross lipemia that cannot be cleared by ultracentrifugation
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 3 days |
| Refrigerated | 14 days |
| Frozen | 14 days |