Hepatitis B Virus (HBV) Patient Management
Also known as: Hepatitis
Use
This panel is intended for use in the management of patients with chronic hepatitis B and to assess the efficacy of antiviral treatment. A positive HBsAg test will reflex to HBeAg, anti-HBe, HBV quantitative DNA, and ALT.
Special Instructions
Not provided.
Limitations
The anti-HBe assay has not been validated for children under 17, pregnant women, or immunocompromised patients. The HBV quantitative real-time PCR assay's sensitivity range is 10 to 1,000,000,000 IU/mL. Interference from high dose biotin supplementation is a concern, and specimens must not be heat-inactivated or collected inadequately to ensure accurate results.
Methodology
Immunoassay (CLIA)
Biomarkers
LOINC Codes
- 5196-1 - HBV surface Ag SerPl Ql IA
- 7905-3 - HBV surface Ag SerPl Ql Nt
- 19147-8 - Ref Lab Test Ref Range
- 1742-6 - ALT SerPl-cCnc
- 13954-3 - HBV e Ag SerPl Ql IA
- 13953-5 - HBV e Ab SerPl Ql IA
- 42595-9 - HBV DNA SerPl NAA+probe-aCnc
- 48398-2 - HBV DNA SerPl NAA+probe-Log IU
Result Turnaround Time
1-7 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
9.5 mL (2.5 mL for HBV DNA)
Minimum Volume
3.6 mL (0.7 mL for HBV DNA)
Container
Gel-barrier tube or red-top tube
Collection Instructions
Two samples must be submitted. For all tests except DNA, if not using a gel-barrier tube, transfer the separated serum to a plastic transport tube. For HBV DNA, centrifuge within 24 hours of collection and transfer to a screw-cap polypropylene transport tube before freezing.
Patient Preparation
Patients should stop biotin consumption 72 hours prior to collection.
Storage Instructions
For all tests except DNA: Refrigerated preferred for up to 7 days, frozen up to 14 days. For HBV DNA: Frozen preferred up to 12 weeks, refrigerated up to 6 days. Room temperature stability varies.
Causes for Rejection
Heat-inactivated specimens; cord blood; cadaveric specimens; body fluid other than serum or EDTA plasma; gross hemolysis; excessive lipemia; improper labeling
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Up to 7 days for all except DNA, 24 hours for DNA |
| Refrigerated | Up to 7 days for all except DNA, up to 6 days for DNA |
| Frozen | Up to 14 days for all except DNA, up to 12 weeks for DNA |
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