Leukocyte Adhesion Deficiency (LAD), Evaluation (CD15s, CD18), Flow Cytometry
Use
Leukocyte adhesion deficiency (LAD) is an autosomal recessive immunodeficiency disorder associated with recurrent bacterial and fungal infections. Two forms are described: LAD‑1, due to defective expression of CD18 on neutrophils, and LAD‑2, due to defective expression of CD15s (sialyl‑Lewis X) ([jdos.nicholsinstitute.com](https://jdos.nicholsinstitute.com/dos/LabFirst/test/160582?utm_source=openai))
Special Instructions
Not provided.
Limitations
Not provided.
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
5 mL
Minimum Volume
2 mL
Container
Sodium heparin (green‑top) tube
Collection Instructions
Collect 5 mL of whole blood in a sodium heparin (green‑top) tube. Mix thoroughly. Transport at room temperature immediately.
Causes for Rejection
Hemolysis • Lipemia • Icterus • Clotted specimens • Received refrigerated • Frozen specimens • Coagulants other than sodium heparin
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | Unacceptable |
| Frozen | Unacceptable |