Prostate Triple Stain (P504S, HMW Keratins, P63), IHC without Interpretation
Also known as: CK14, P504S, Prostate Cancer, CK5, 34betaE12
Use
This immunohistochemical (IHC) stain cocktail targets three markers—P504S (AMACR), high molecular weight keratins (HMW KRT), and p63—used to aid in differentiating benign prostate gland basal cells from malignant prostate epithelial cells, and in distinguishing high‑grade prostatic intraepithelial neoplasia (PIN) and prostate adenocarcinoma. The absence of basal cell staining (p63, HMW KRT) and the presence of P504S in epithelial cells supports a diagnosis of carcinoma. This test is used when pathological evaluation of small or limited prostate specimens is challenging.
Special Instructions
This version of the test includes only the technical performance of immunostaining without pathologist interpretation. Clients wishing to receive interpretation must order the companion 'with Interpretation' test (test code 91478).
Limitations
Interpretation of staining results must be conducted by a qualified pathologist in the clinical and morphological context. Technical factors such as age of paraffin section (best if cut within 6 weeks), slide charge, humidity, and storage conditions may impact staining quality. Unsuitable slides or blocks may yield inconsistent results.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Formalin‑fixed, paraffin‑embedded tissue block OR 2 unstained, positively charged glass slides (25×75×1 mm), 4‑micron sections
Collection Instructions
Submit formalin‑fixed, paraffin‑embedded tissue block, or 2 unstained, positively charged slides, sections 4‑microns thick
Causes for Rejection
Wet/frozen tissue; cytology smears; non‑formalin fixed tissue; non‑paraffin embedded tissue; non‑charged slides; ProbeOn slides; Snowcoat slides