Melan A by Immunohistochemistry (DAB Detection)
Also known as: MELANA IHC
Use
Immunohistochemical stain for Melan‑A (MART‑1) used for detection of melanocytic differentiation—commonly applied in evaluation of melanoma and other melanocyte‑lineage tumors. This stain aids in confirming diagnosis when morphological features are uncertain.
Special Instructions
This test is performed as a stain‑and‑return (technical) service only. Electronic request submission is preferred; if unavailable use ARUP Immunohistochemistry Stain Form #32978 with an ARUP client number.
Limitations
Specimens must be adequate (not depleted or non‑representative) and properly fixed in 10% neutral buffered formalin, paraffin‑embedded, or cell‑block prepared. Precut slides should not be oven‑baked. Frozen specimens unacceptable. Interpretation depends on staining quality.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 94736‑6
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
tissue block or 5 unstained (3‑ to 5‑micron thick) positively charged slides
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen (cells must be prepared into a cellblock). Protect paraffin block and/or slides from excessive heat. Transport tissue block or 5 unstained (3‑ to 5‑micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808). If sending precut slides, do not oven bake.
Storage Instructions
Room temperature or refrigerated; ship in cooled container during summer months.
Causes for Rejection
Non‑representative tissue type; depleted specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |