Oncology FISH Analysis - TEL/AMLI: t(12;21) [ALL]
Use
Oncology FISH Analysis - TEL/AMLI: t(12;21) is a Fluorescence In Situ Hybridization (FISH) test designed to detect translocation between 12p13 and 21q22, which fuses the N-terminus of TEL with the AML1 gene. The TEL/AML1 fusion is associated with acute lymphoblastic leukemia (ALL). This translocation is noted in approximately 25% of B-cell childhood ALL cases and 5% of adult ALL cases, indicating a favorable prognosis. Results must be interpreted in the context of clinical and pathological data, and the test should not be relied upon alone for diagnosis.
Special Instructions
This assay is particularly useful for patients who either exhibit clinical signs of acute lymphoblastic leukemia (ALL) or where prior cytogenetic testing has failed. Additionally, it serves a vital role in monitoring treatment responses and identifying prognostic anomalies in diagnosed patients. Shipping conditions are strict, and specimens must be sent in insulated containers to maintain ambient temperature, arriving within a specified timeframe for optimal results.
Limitations
This FISH assay only identifies the specific translocation of the TEL gene on 12p13 and AML1 gene on 21q22.12 and cannot determine translocations involving other partner chromosomes. Therefore, other potential genetic abnormalities may not be detected.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
4 days
Related Documents
For more information, please review the documents below
Specimen
Bone Marrow (Fresh)
Volume
3-5 cc (Adults/Children) or 2 cc (Infants < 2 years)
Minimum Volume
2 cc
Container
Sodium Heparin (green-top) tube
Collection Instructions
Draw bone marrow in a Sodium Heparin (green-top) tube(s)
Storage Instructions
Ship at ambient temperature (18-25°C/64-77°F) in an insulated container by overnight courier.
Causes for Rejection
Do not heat or freeze.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hrs |