Calponin by Immunohistochemistry
Also known as: CALPON IHC
Use
Calponin is a smooth muscle–specific cytoskeletal protein useful in identifying smooth muscle tumors and myoepithelial cells, such as demonstrating the presence or absence of myoepithelium in breast lesions. It is performed as a stain-and-return (technical) immunohistochemistry service.
Special Instructions
Submit electronic request or use ARUP Immunohistochemistry Stain Form (#32978) with a client number. Ordering recommendation refers to ARUP Immunohistochemistry Stain Offerings brochure. This is a stain-and-return technical service.
Limitations
This test is performed as a stain-and-return (technical) service only. It may be limited by specimen quality, representativeness, depleted specimens, or non-representative tissue types. Precut slides must not be oven baked.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 94736-6
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Min: 2 slides
Container
tissue transport kit (ARUP supply #47808)
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen; cells must be prepared into a cellblock. Protect paraffin block and/or slides from excessive heat. Transport tissue block or 5 unstained (3- to 5‑micron thick sections), positively charged slides. If sending precut slides, do not oven bake.
Storage Instructions
Room temperature or refrigerated; ship in cooled container during summer months.
Causes for Rejection
Non‑representative tissue type; depleted specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |