Oncology FISH Analysis - TCF3/PBX1 FISH for ALL

Clinical Use

Use

The Oncology FISH Analysis - TCF3/PBX1 is designed to detect the translocation between the TCF3 and PBX1 genes, specifically t(19p13.3;1q23.3). This fusion is known to be associated with pre-B-cell acute lymphoblastic leukemia (B-ALL), carrying adverse prognostic implications. The results of this FISH assay must always be interpreted along with other relevant clinical and pathological data, as it should not be relied upon solely for diagnosing disease. It is particularly indicated for patients suspected of having B-ALL, for the prognostic identification of anomalies in known patients, and in cases where cytogenetic efforts have failed. Furthermore, it aids in assessing treatment responses.

Special Instructions

To order the test, follow the respective guidelines in the test catalog or use the order kits link. Shipping must be arranged carefully, ensuring the specimens reach the laboratory within optimal timeframes. Follow the instructions for specimen handling closely to preserve sample integrity. Monitor for any potential delays in shipping, particularly in adverse weather conditions, and communicate with the laboratory as needed. Ensure compliance with local regulations regarding specimen transport when submitting samples.

Limitations

This assay is specifically designed to only identify the translocation involving the TCF3 gene located on 19p13.3 and the PBX1 gene located on 1q23.3. It cannot detect translocations involving any other partner chromosomes, which limits the scope of this analysis. Results should be interpreted in the clinical context as they do not provide a full picture of the underlying leukemia or other related conditions.

Test Details