FISH — Chromosomes 4, 10 & 17 enumeration
Use
Fluorescence in situ hybridization (FISH) enumeration for chromosomes 4, 10, and 17 is used to identify trisomies of these chromosomes, most commonly in the context of pediatric acute lymphoblastic leukemia (ALL). Trisomies of chromosomes 4, 10, and/or 17 serve as prognostic indicators, assist with monitoring disease course, and may detect measurable residual disease. This test is used as an adjunct to conventional cytogenetic and clinical information in diagnosing and managing B-cell ALL.
Special Instructions
Not provided.
Limitations
Negative results do not rule out the presence of a neoplastic disorder. Interpretation must be made within the context of other clinical and pathologic information. This assay is not approved by the FDA and should be used as adjunctive information. It may not detect structural or numerical abnormalities beyond trisomies of chromosomes 4, 10, and 17.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
7-10 days
Related Documents
For more information, please review the documents below
Specimen
Bone Marrow
Volume
2 mL
Minimum Volume
1 mL
Container
Sodium heparin (green top tube)
Storage Instructions
20‑25 °C for transport; must arrive within 24 hours
Causes for Rejection
Frozen or clotted specimens; specimens in anticoagulants other than sodium heparin
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 24 hours |
| Frozen | unacceptable |